Resources

The Lindstrom Lab – Kidney Organoids

The Quadrato Lab – Brain Organoids

Stem Cell Culture Protocols:

1. Donning and Doffing Personal Protective Equipment (PPE)

2. Basics of Aseptic Technique (setting up to work in a BioSafety Cabinet)

3. Media Preparation (Making complete media)

4. Aliquoting Matrigel

5. Plate Coating (Matrigel)

6. Stem Cell Quick Thaw

7. Stem Cell Feeding & Maintenance

8. Stem Cell Passaging (EDTA)

Organoid Differentiation Protocols:

• Kidney organoid differentiation

• Forebrain organoid differentiation

Single Nuclei Sequencing Protocols:

• Manual isolation of nuclei from snap frozen samples

• Computational pipelines for snRNA-seq and snATAC-seq

 

The USC ASCEND SRL is part of a larger network of CIRM (California Institute of Regenerative Medicine) funded SRLs with a shared goal of democratizing stem cell research tools, standardizing methodologies, and facilitating interdisciplinary collaborations. Links to those institutions and their services can be found via CIRM.