These courses are designed for participants interested in learning about more specialized research methods. Each advanced course will include background information on the tools and techniques, as well as resources for experimental design and data analysis.

Target Audience: Individuals proficient in cell culture

Gene Editing (2 days, $1,000): In this course, participants will cover concepts of CRISPR/Cas9 gene editing and recent advances in gene modification methods. The course covers:

• defining CRISPR components
• designing guide RNAs
• CRISPR delivery methods
• evaluating gene targeting efficiency
• introduction to Cas9 fusion proteins
• introduction to CRISPR screening

Tissue Engineering (2 days, $1,000): In this course, participants will learn about the integration of stem cells into biomaterials for the purpose of modeling complex tissues and organs. The course covers:

• scaffolds and biocompatible materials
• 3D printing techniques
• cell seeding onto biomaterials
• tissue engineering applications
• microfluidic devices and organ-on-a-chip
• functional assays of engineered tissues

Spatial Transcriptomics (2 days, $1,000): In this course, participants will cover the basics for designing a spatial transcriptomics experiment and evaluating the data. The course covers:

• experimental design
• equipment and reagents
• sample preparation and cryosectioning
• machine operation and data management
• introduction to data analysis
• tools for data representation

Single Cell Sequencing Data Analysis (2 days, $1,000): Participants will cover the basic methods for evaluating single cell genomic and transcriptomic data. The course covers:

• sample data sets (post-alignment)
• Seurat analysis in R
• data comparison and integration
• customized advanced analysis
• code documentation

Cell and Gene Therapy (5 days, $2,000): Participants will explore the translation of stem cell research into clinical applications. The course covers:

• therapeutic approaches
• manufacturing cell and gene therapy products
• clinical trial design
• FDA regulations and approval
• case studies on current clinical trials
• FDA approved cell and gene therapies
• ethical considerations for regenerative medicine